Total Red Blood Cell Count and Differential White Blood Cell Count Experiment
done using some tool to get real value with units, precisely measure exact amount with special tool
Purpose of Total RBC Count
determine concentration of Red Blood Cells in a sample
What is the healthy range of RBC?
slide with grid printed on it
How do you get a countable number?
dilute the sample
how much the sample is diluted and what you use to multiply with the countable number to achieve total RBC count
Determine relative amount of 5 classes of White Blood Cell
Purpose of Differential White Blood Cell Count
Why do we use Total RBC Count?
to diagnose blood conditions
Too many red blood cells
too few red blood cells
why do we count WBC?
to diagnose WBC conditions
What is the condition in which a person has too many of one class of cell type?
what are the 5 types of leukocytes?
neutrophils, basophils, eosinophils, monocytes, lymphocytes
predominantly trilobed nucleus
healthy range of the amount of neutrophils in a sample?
Big red cytoplasmic granules and bilobed
Usually, how many eosinophils are in a healthy sample?
100-400 cells / microliters
Dark cytoplasmic granules, least abundant
How many basophils are there in a healthy sample?
20-50 cells / microliter
what are the two types of leukocytes?
granulocytes and agranulocytes
contain granules in cytoplasm of cells
do not contain cytoplasmic granules
smallest, rim of cytoplasm around nucleus
how many lymphocytes are usually present in a sample?
1500-3000 cells / microliter
puffy U-shaped nucleus and is very large
How often can you see a monocyte in a healthy sample?
100-700 cells / microliter