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Total Red Blood Cell Count and Differential White Blood Cell Count Experiment

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1

Quantitative

done using some tool to get real value with units, precisely measure exact amount with special tool

2

Qualitative

relative amounts

3

Purpose of Total RBC Count

determine concentration of Red Blood Cells in a sample

4

What is the healthy range of RBC?

4-6million RBC/microliter

5

slide with grid printed on it

hemocytometer

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How do you get a countable number?

dilute the sample

7

dilution factor

how much the sample is diluted and what you use to multiply with the countable number to achieve total RBC count

8

Determine relative amount of 5 classes of White Blood Cell

Purpose of Differential White Blood Cell Count

9

Why do we use Total RBC Count?

to diagnose blood conditions

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Too many red blood cells

polycythemia

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too few red blood cells

anemia

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why do we count WBC?

to diagnose WBC conditions

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What is the condition in which a person has too many of one class of cell type?

leukemia

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what are the 5 types of leukocytes?

neutrophils, basophils, eosinophils, monocytes, lymphocytes

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predominantly trilobed nucleus

neutrophil

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healthy range of the amount of neutrophils in a sample?

3000-7000 cells/microliter

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Big red cytoplasmic granules and bilobed

Eosinophils

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Usually, how many eosinophils are in a healthy sample?

100-400 cells / microliters

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Dark cytoplasmic granules, least abundant

Basophils

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How many basophils are there in a healthy sample?

20-50 cells / microliter

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what are the two types of leukocytes?

granulocytes and agranulocytes

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granulocytes

contain granules in cytoplasm of cells

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agranulocytes

do not contain cytoplasmic granules

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smallest, rim of cytoplasm around nucleus

lymphocytes

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how many lymphocytes are usually present in a sample?

1500-3000 cells / microliter

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puffy U-shaped nucleus and is very large

monocyte

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How often can you see a monocyte in a healthy sample?

100-700 cells / microliter

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artifact

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