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Exercise 2-2 Micro Lab: Colony Morphology

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1

How does a colony form?

When a single bacterial cell is deposited on a solid nutrient medium, it begins to divide. One cell makes two, two makes four, four make eight. Eventually a visible mass of cells, a colony, appears.

2

In what 4 ways are color, size, shape, and texture of microbial growth determined?

1. genetic makeup of the organism
2. nutrient availability
3. temperature
4. incubation

3

The 5 basic categories of colony morphology

1. colony shape
2. margin (edge)
3. elevation
4. texture
5. pigment production (color)

4

3 types of colony shape

1. round
2. irregular
3. punctiform (tiny, pinpoint)

5

5 types of margin

1. entire (smooth with no irregularities)
2. undulate (wavy)
3. lobate (lobed)
4. filamentous
5. rhizoid (branched like roots)

6

entire

smooth with no irregularities

7

undulate

wavy

8

lobate

lobed

9

rhizoid

branched like roots

10

5 elevations of colonies

1. flat
2. raised
3. convex
4. pulvinate (very convex)
5. umbonate (raised in the center)

11

pulvinate

very convex

12

umbonate

raised in the center

13

3 types of texture

1. moist
2. mucoid
3. dry

14

4 types of pigment production (color)

1. opaque
2. translucent
3. shiny
4. dull

15

colony counter

used to view subtle differences in colony shape and size

16

what 2 things allow greater observation of detail in a colony counter?

1. transmitted light
2. magnifying glass

17

colony counter is best determined with ______.

reflected light

18

The grid in the colony counter background is a ______.

counting aid

19

6 colonies studied in this lab

1. micrococcus luteus
2. corynebacterium xerosis
3. lactobacillus plantarum
4. mycobacterium smegmatis
5. bacillus subtilis
6. proteus miabilis

20
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Name these elevations, left to right

1. raised
2. raised, spreading edge
3. flat, raised margin
4. growth into medium

21
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Name these elevations, left to right

1. convex
2. umbonate
3. plateau
4. flat

22
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Name these margins, left to right

1. smooth, entire
2. rhizoid
3. irregular (erose)
4. lobate
5. filamentous

23

3 types of descriptions used to describe colonial morphology:

1. color
2. surface characteristics
3. consistency
4. optical properties

24

2 types of surface characteristics

1. dull
2. shiny

25

3 types of consistency

1. dry
2. butyrous-buttery
3. moist

26

2 types of optical properties

1. opaque
2. translucent

27
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What bacteria is this?
What is the color, shape, elevation and margin?

Staphylococcus epidermidis

Color - white
Shape - circular
Elevation - raised
Margin - entire

28
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What bacteria is this?

What 3 features that describe it?

Providencia stuartii

1. shiny
2. buff
3. convex

29
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What bacteria is this?

What 3 features that describe it?

Klebsiella pneumoniae

1. mucoid
2. raised
3. shiny

30
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What bacteria is this?

What 3 features that describe it?

Chromobacterium violaceum

1. shiny
2. purple
3. convex

31
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What bacteria is this?

What is the color, shape, elevation and margin of it?

Enterococcus faecium

Color - white
Shape - circular
Elevation - Convex
Margin - Entire

32
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What is this?

5 features of the colonies

A) Bacillus cereus
2) Bacillus anthracis

1. dull
2. dry
3. raised
4. rough-textured
5. gray

33
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What is being shown in these two views? What bacteria is this? What are the 3 features of this bacteria?

A) Alcaligenes Faecalis side view showing a raised center

B) Alcaligenes Faecalis showing spreading edge

1. umbonate
2. opaque center
3. spreading edge

34
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What is being shown here?

Filamentous growth

35
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What bacteria is being shown in A and B and what are the differences between them?

What 2 features of the bacteria are being showed here?

Where is this bacteria found?

A) Clostridium sporaogenes grown on sheep blood agar and viewed with reflected light

B) Clostridium sporaogenes grown on nutrient agar and viewed with transmitted light

1. irregular
2. rhizoid

It is found in soil

36

_____ is an opportunistic pathogen

Staphylococcus epidermidis

37

______ is a frequent isolate in urine samples obtained from hospitalized and catheterized patients

Providencia stuartii

38

______ is found in human and animal feces

Enterococcus faecium

39
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What bacteria is this?

What does this growth demonstrate?

Alcaligenes faecalis

Demonstrates spreading attributable to motility and is translucent

40

_______ is found in soil and water and rarely produces infections in humans

Chromobacterium violaceum

41
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What are these slides showing?

What bacteria is this?

The effect of age on colony morphology after 24 and 48 hours of growth

Bacillus subtilis

A) After 24 hours
B) After 48 hours (note worm like appearance)

42
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What bacteria is this?

This bacteria produces colonies with what 2 features?

Bacillus subtilis

1. raised margin
2. dull surface

43
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what bacteria is this?

what feature of this colony is being shown here?

Mycobacterium smegmatis

punctiform

44
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What bacteria is this?

In A, what 4 features of the bacteria are exhibited?

What is being shown here in B?

Coynebacterium xerosis

In A:

1. round
2. dull
3. buff
4. convex

In B: circular

45
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What bacteria is this?

What is being demonstrated in this dish?

Erwinia amylovora

the irregular shape and spreading edges

46

_________ is a plant pathogen.

Erwinia amylovora

47
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What bacteria is shown here?

What is this slide demonstrating?

Proteus vulgaris

swarming growth pattern due to bacteria motility

48
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What bacteria is shown here?

What is being demonstrated here?

Pseudomonas aeruginosa

Mucoid texture

49

_____________ is found in soil and water and can cause infections in burn patients

Pseudomonas aeruginosa

50
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What are these samples showing?

Mixed soil cultures and diversity

51
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What bacteria is this showing? How do we know?

Staphylococcus aureus

White growth on blood agar plate demonstrating B-hemolysis is characteristic of staph. In A) the agar is darkening from the hemolysis. In B) there is weak hemolysis.

52
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What bacteria is this?

Chromobacterium violaceum

53
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What bacteria is on the left? the right?

What is being demonstrated here?

Left - Micrococcus luteus
Right - Kocuria rosea

Pigment production in similar species of bacteria

54
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What bacteria is this?

What is being shown here with the encircled sections?

Serratia marcescens

the influence of age on pigment production after 24 and 48 hours

55
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What bacteria is being shown here?

What is being demonstrated here?

Serratia marcescens

influence of temperature on pigment production when grown in 37 degrees C on left and 25 degrees C on right

56
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What bacteria is being shown here?

What is being demonstrated here?

Chromobacterium violaceum

The influence of nutrient availability on pigment production. The nutrient agar (right) has less nutrients so less pigment.

57
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What bacteria is shown here?

What is being demonstrated here?

Pseudomonas aeruginosa

diffusible blue-green pigment


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